This question should be on Server Fault. Nevertheless, the following should do the trick, assuming you're talking about TCP and the IP you want to allow is 1.2.3.4:
iptables -A INPUT -p tcp --dport 8000 -s 1.2.3.4 -j ACCEPT
iptables -A INPUT -p tcp --dport 8000 -j DROP
sudo iptables -A INPUT -p tcp --dport 8000 -s ! 1.2.3.4 -j DROP
I had similar issue that 3 bridged virtualmachine just need access eachother with different combination, so I have tested this command and it works well.
Edit**
According to Fernando comment and this link exclamation mark
(!) will be placed before than -s parameter:
sudo iptables -A INPUT -p tcp --dport 8000 ! -s 1.2.3.4 -j DROP