I'm not a mod_rewrite expert and often struggle with it, but I have done this on one of my sites. It might need other flags, etc., depending on your circumstances. I'm using this:
You can use the following rule in .htaccess to rewrite a subdomain to a subfolder:
RewriteEngine On
# If the host is "sub.domain.example"
RewriteCond %{HTTP_HOST} ^sub.domain.example$ [NC]
# Then rewrite any request to /folder
RewriteRule ^((?!folder).*)$ /folder/$1 [NC,L]
Line-by-line explanation:
RewriteEngine on
The line above tells the server to turn on the engine for rewriting URLs.
This line is a condition for the RewriteRule where we match against the HTTP host using a regex pattern. The condition says that if the host is sub.domain.example then execute the rule.
RewriteRule ^((?!folder).*)$ /folder/$1 [NC,L]
The rule matches http://sub.domain.example/foo and internally redirects it to http://sub.domain.example/folder/foo.
Replace sub.domain.example with your subdomain and folder with name of the folder you want to point your subdomain to.